Lysis:Article Title: MicroRNA gene dynamics in immune cell subpopulations during aging and atherosclerosis disease development at single-cell resolution.
Article Snippet: .. Lysis buffer (1 ml per 100 mg of tissue) was freshly prepared, containing a low-salt/homogenization buffer (20 mM HEPES pH 7.4; Fisher, 10,041,703), 150 mM KCl (Sigma, 60,142), 10 mM MgCl2 (Invitrogen, AM9530G), supplemented with 0.5 mM dithiothreitol (DTT) (Sigma, 10,197,777,001), cOmplete Mini EDTA-free protease inhibitor (Roche 11,836,170,001, one mini tablet/10 ml), 100 μg/ml cycloheximide (Sigma-Aldrich, 1810) in dimethylsulfoxide (DMSO) (Sigma C1988-1G), 0.2 U/μl murine RNase inhibitor (NEB M0314L), and 0.1 U/μl Superasin RNase inhibitor (Thermo Scientific AM2696). .. The homogenized samples were centrifuged at 2000 × g for 10 min at 4 °C and the supernatant was collected, mixed with 10% IGEPAL CA630 (Sigma, I8896)] to reach final 1%v/v and with 1,2-diheptanoyl-sn-glycero-3-phosphocholine (Avanti Polar Lipids, 850306P) in final concentration of 30 mM.
Article Title: MicroRNA gene dynamics in immune cell subpopulations during aging and atherosclerosis disease development at single-cell resolution.
Article Snippet: The bead-protein complex was used to capture anti-GFP antibodies (clone 19F7 and clone 19C8, from the Antibody and Bioresource Core Facility at Memorial Sloan Kettering Cancer Center). .. Lysis buffer was freshly prepared, containing a low-salt/homogenization buffer (20 mM HEPES pH 7.4; Fisher, 10,041,703), 150 mM KCl (Sigma, 60,142), 10 mM MgCl2 (Invitrogen, AM9530G), supplemented with 0.5 mM DTT (Sigma, 10,197,777,001), cOmplete Mini EDTA-free protease inhibitor (Roche 11,836,170,001, one mini tablet/10 ml), 100 μg/ml cycloheximide (Sigma-Aldrich, 1810) in dimethylsulfoxide DMSO (Sigma C1988-1G), 0.2 U/μl murine RNase inhibitor (NEB M0314L), and 0.1 U/μl Superasin RNase inhibitor (Thermo Scientific AM2696). ..
Article Title: Single-cell to pre-clinical evaluation of Trem2, Folr2, and Slc7a7 as macrophage-associated biomarkers for atherosclerosis.
Article Snippet: Tissue was frozen in liquid nitrogen, pulverized using a Cellcrusher cryo-press (Cellcrusher, 9 Cork, Ireland) and further processed in Lysis buffer with Dounce homogenizer. .. Lysis buffer was 10 freshly prepared, containing a low-salt/homogenization buffer (20 mM HEPES pH 7.4; Fisher, 11 10041703), 150 mM KCl (Sigma, 60142), 10 mM MgCl2 (Invitrogen, AM9530G), supplemented 12 with 0.5 mM dithiothreitol (DTT) (Sigma, 10197777001), cOmplete Mini EDTA-free protease 13 inhibitor (Roche 11836170001, one mini tablet/10 mL), 100 μg/mL cycloheximide (Sigma-14 Aldrich, 1810) in dimethylsulfoxide (DMSO) (Sigma C1988-1G), 0.2 U/μL murine RNase 15 inhibitor (NEB M0314L), and 0.1 U/μL Superasin RNase inhibitor (Thermo Scientific AM2696). ..
Article Title: MicroRNA gene dynamics in immune cell subpopulations during aging and atherosclerosis disease development at single-cell resolution
Article Snippet: The bead-protein complex was used to capture anti-GFP antibodies (clone 19F7 and clone 19C8, from the Antibody and Bioresource Core Facility at Memorial Sloan Kettering Cancer Center). .. Lysis buffer was freshly prepared, containing a low-salt/homogenization buffer (20 mM HEPES pH 7.4; Fisher, 10,041,703), 150 mM KCl (Sigma, 60,142), 10 mM MgCl2 (Invitrogen, AM9530G), supplemented with 0.5 mM DTT (Sigma, 10,197,777,001), cOmplete Mini EDTA-free protease inhibitor (Roche 11,836,170,001, one mini tablet/10 ml), 100 μg/ml cycloheximide (Sigma-Aldrich, 1810) in dimethylsulfoxide DMSO (Sigma C1988-1G), 0.2 U/μl murine RNase inhibitor (NEB M0314L), and 0.1 U/μl Superasin RNase inhibitor (Thermo Scientific AM2696). ..
Article Title: MicroRNA gene dynamics in immune cell subpopulations during aging and atherosclerosis disease development at single-cell resolution
Article Snippet: .. Lysis buffer (1 ml per 100 mg of tissue) was freshly prepared, containing a low-salt/homogenization buffer (20 mM HEPES pH 7.4; Fisher, 10,041,703), 150 mM KCl (Sigma, 60,142), 10 mM MgCl 2 (Invitrogen, AM9530G), supplemented with 0.5 mM dithiothreitol (DTT) (Sigma, 10,197,777,001), cOmplete Mini EDTA-free protease inhibitor (Roche 11,836,170,001, one mini tablet/10 ml), 100 μg/ml cycloheximide (Sigma-Aldrich, 1810) in dimethylsulfoxide (DMSO) (Sigma C1988-1G), 0.2 U/μl murine RNase inhibitor (NEB M0314L), and 0.1 U/μl Superasin RNase inhibitor (Thermo Scientific AM2696). .. The homogenized samples were centrifuged at 2000 × g for 10 min at 4 °C and the supernatant was collected, mixed with 10% IGEPAL CA630 (Sigma, I8896)] to reach final 1%v/v and with 1,2-diheptanoyl-sn-glycero-3-phosphocholine (Avanti Polar Lipids, 850306P) in final concentration of 30 mM.
Protease Inhibitor:Article Title: MicroRNA gene dynamics in immune cell subpopulations during aging and atherosclerosis disease development at single-cell resolution.
Article Snippet: .. Lysis buffer (1 ml per 100 mg of tissue) was freshly prepared, containing a low-salt/homogenization buffer (20 mM HEPES pH 7.4; Fisher, 10,041,703), 150 mM KCl (Sigma, 60,142), 10 mM MgCl2 (Invitrogen, AM9530G), supplemented with 0.5 mM dithiothreitol (DTT) (Sigma, 10,197,777,001), cOmplete Mini EDTA-free protease inhibitor (Roche 11,836,170,001, one mini tablet/10 ml), 100 μg/ml cycloheximide (Sigma-Aldrich, 1810) in dimethylsulfoxide (DMSO) (Sigma C1988-1G), 0.2 U/μl murine RNase inhibitor (NEB M0314L), and 0.1 U/μl Superasin RNase inhibitor (Thermo Scientific AM2696). .. The homogenized samples were centrifuged at 2000 × g for 10 min at 4 °C and the supernatant was collected, mixed with 10% IGEPAL CA630 (Sigma, I8896)] to reach final 1%v/v and with 1,2-diheptanoyl-sn-glycero-3-phosphocholine (Avanti Polar Lipids, 850306P) in final concentration of 30 mM.
Article Title: MicroRNA gene dynamics in immune cell subpopulations during aging and atherosclerosis disease development at single-cell resolution.
Article Snippet: The bead-protein complex was used to capture anti-GFP antibodies (clone 19F7 and clone 19C8, from the Antibody and Bioresource Core Facility at Memorial Sloan Kettering Cancer Center). .. Lysis buffer was freshly prepared, containing a low-salt/homogenization buffer (20 mM HEPES pH 7.4; Fisher, 10,041,703), 150 mM KCl (Sigma, 60,142), 10 mM MgCl2 (Invitrogen, AM9530G), supplemented with 0.5 mM DTT (Sigma, 10,197,777,001), cOmplete Mini EDTA-free protease inhibitor (Roche 11,836,170,001, one mini tablet/10 ml), 100 μg/ml cycloheximide (Sigma-Aldrich, 1810) in dimethylsulfoxide DMSO (Sigma C1988-1G), 0.2 U/μl murine RNase inhibitor (NEB M0314L), and 0.1 U/μl Superasin RNase inhibitor (Thermo Scientific AM2696). ..
Article Title: MicroRNA gene dynamics in immune cell subpopulations during aging and atherosclerosis disease development at single-cell resolution
Article Snippet: The bead-protein complex was used to capture anti-GFP antibodies (clone 19F7 and clone 19C8, from the Antibody and Bioresource Core Facility at Memorial Sloan Kettering Cancer Center). .. Lysis buffer was freshly prepared, containing a low-salt/homogenization buffer (20 mM HEPES pH 7.4; Fisher, 10,041,703), 150 mM KCl (Sigma, 60,142), 10 mM MgCl2 (Invitrogen, AM9530G), supplemented with 0.5 mM DTT (Sigma, 10,197,777,001), cOmplete Mini EDTA-free protease inhibitor (Roche 11,836,170,001, one mini tablet/10 ml), 100 μg/ml cycloheximide (Sigma-Aldrich, 1810) in dimethylsulfoxide DMSO (Sigma C1988-1G), 0.2 U/μl murine RNase inhibitor (NEB M0314L), and 0.1 U/μl Superasin RNase inhibitor (Thermo Scientific AM2696). ..
Article Title: MicroRNA gene dynamics in immune cell subpopulations during aging and atherosclerosis disease development at single-cell resolution
Article Snippet: .. Lysis buffer (1 ml per 100 mg of tissue) was freshly prepared, containing a low-salt/homogenization buffer (20 mM HEPES pH 7.4; Fisher, 10,041,703), 150 mM KCl (Sigma, 60,142), 10 mM MgCl 2 (Invitrogen, AM9530G), supplemented with 0.5 mM dithiothreitol (DTT) (Sigma, 10,197,777,001), cOmplete Mini EDTA-free protease inhibitor (Roche 11,836,170,001, one mini tablet/10 ml), 100 μg/ml cycloheximide (Sigma-Aldrich, 1810) in dimethylsulfoxide (DMSO) (Sigma C1988-1G), 0.2 U/μl murine RNase inhibitor (NEB M0314L), and 0.1 U/μl Superasin RNase inhibitor (Thermo Scientific AM2696). .. The homogenized samples were centrifuged at 2000 × g for 10 min at 4 °C and the supernatant was collected, mixed with 10% IGEPAL CA630 (Sigma, I8896)] to reach final 1%v/v and with 1,2-diheptanoyl-sn-glycero-3-phosphocholine (Avanti Polar Lipids, 850306P) in final concentration of 30 mM.
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